Responses of inflammation signaling pathway by saucerneol D from elicitor-treated Saururus chinensis on pro-inflammatory responses in LPS-stimulated Raw 264.7 cell

Abstract This study confirmed the association with inflammation-related proteins, mediators, and cytokines using saucerneol D from Saururus chinensis leaf, a useful ingredient increased through elicitor treatment. To confirm anti-inflammatory effect, were treated lipopolysaccharide, which induces pro-inflammatory factors in Raw 264.7 cell. The influences measured by dint of chemical assay western blotting as well ELISA. As result, content was changed when eicitor various concentration (1.5, 3 mg/mL) S. leaves. In addition, expression levels hyaluronidase pro-inflammatory-related [nitric oxide (NO), inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2)] regulated according to elicitor-treated non-treated groups. Therefore, after confirming that has an inhibitory effect on factors, adjusted compared control substance verify efficacy. Saucerneol did not toxic macrophages 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. controlled at concentrations inhibited iNOS COX-2 proteins. produced activity, prostaglandin E 2 (PGE ), inflammatory mediator [interleukin-1β (IL-1β), IL-6, tumor necrosis factor-α (TNF-α)] significantly suppressed. it D, active treatment, could be used functional material controls factors.